Tissue Culture Regeneration of a Medicinal Plant from Mexico: Piper auritum Kunth.
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Tissue Culture Regeneration of a Medicinal Plant from Mexico: Piper auritum Kunth
An effi cient whole plant regeneration method from callus cultures of Piper auritum was achieved through organogenesis derived from leaf tissue. Proliferating callus and shoot cultures derived from leaf tissue explants placed on Murashige and Skoog (MS) medium supplemented with 2.0 mg·L 2, 4-dichlorophenoxyacetic acid (2,4-D) plus 1.5 mg·L kinetin. Optimum combination of hormones (mg·L) for sho...
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An in vitro regeneration protocol through stem callus culture has been standardized for the medicinal climber Clematis gouriana. The explant induced callus on MS-medium supplemented with 0.5 to 1.5 mg l BAP and 0.1 to 0.5 mg l NAA. The optimized callus induction occurred at the concentration of 1.0 mg l BAP and 0.3 mg l NAA. After initiation of callus, it was immediately transferred to MS mediu...
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ژورنال
عنوان ژورنال: HortScience
سال: 2006
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.41.1.207